Micron Spherical Silica Gel Powder for Cosmetics Additive and Column Chromatography
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Review of Practical Application of Silica Gel in Column Chromatography(III)

5. Practical application examples of column chromatography silica gel


5.1 Study on the separation of Lanzhou red radish pigments by thin layer chromatography and column chromatography.


Thin layer chromatography and column chromatography were used to separate and analyze the pigments in red radish, focusing on the effects of factors such as the

amount of pigment, eluent, and elution time on pigment separation. We selected better conditions using silica gel as the stationary phase and a mixture of absolute

ethanol/water (5: 1, v/v) as the eluent to conduct chromatographic separation in one direction and at once.


5.2 Preparation of high-purity kaurane diterpenes by atmospheric pressure silica gel column chromatography


High-purity kaurane-type diterpenoids were isolated from half-flag flag extract, and the separation process conditions were established. The appropriate elution mobile

phase was screened by TLC experiment, separated and purified by atmospheric pressure silica gel column chromatography, and the product was recrystallized by the

two-phase method. TLC and MS were used to monitor the separation effect and product purity. The results show that the preferred mobile phase is a mixed solvent of

petroleum ether: acetone: vinegar, 7:3:0.05 (volume ratio), and two kaurane-type diterpene single-molecule compounds are separated after purification.

The purity increased from 62% and 17% to 96% and 94% respectively.


5.3 Separation of Sophora Sophora Alkaloids Series by Reduced Pressure Silica Gel Column Chromatography


Use silica gel column chromatography to purify the alkaloids contained in sophora sophora seeds, using chloroform and methanol as eluents.

By changing the mixing ratio of the two solvents for elution, monomers of matrine, oxymatrine and other alkaloids were obtained. Since there are no standards for other

alkaloids, they are not discussed in this article. The yields of matrine and oxymatrine were 22.2% and 42.6% respectively. After the monomers were tested by HPLC, the

purity reached more than 95%.

Therefore, silica gel column chromatography can be used to refine the alkaloids contained in sophora beans.


5.4 Research on the separation of resveratrol by medium pressure column chromatography


The resveratrol in Polygonum cuspidatum was extracted, separated and determined.

The effects of five factors, including extraction temperature, extraction liquid concentration, extraction time and frequency, and solid-liquid ratio, on the extraction of

resveratrol were investigated.

The optimal extraction conditions for resveratrol were established as follows: extraction liquid fraction is 95%, extraction temperature is 60°C, solid-liquid ratio is 11:1,

and reflux extraction is performed three times, each time for 60 minutes. Medium-pressure silica gel column chromatography was used to separate resveratrol. After

recrystallization, the content of the product was determined to be 99.28% by high-performance liquid chromatography. The results show that the established preparation,

separation and purification process can be used for the production of resveratrol.