Micron Spherical Silica Gel Powder for Cosmetics Additive and Column Chromatography
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Application of Column Chromatography Silica Gel

Column chromatography silica gel is divided into two types: normal phase column chromatography silica gel and reverse column chromatography silica gel. The norDSC_0393.JPGmal phase column chromatography silica gel packing is normal phase silica gel, which is suitable for separating relatively less polar compounds, such as various types of aglycones; Therefore, less polar organic solvents are usually used as eluents, such as petroleum ether-dichloromethane elution system, chloroform-ethyl acetate elution system, etc. Reverse column chromatography silica gel uses reversed-phase silica gel, which is suitable for separating relatively polar compounds, such as alkaloids and other glycosides. Different ratios of methanol-water and acetonitrile-water are often used as eluents.



When loading silica gel for column chromatography, it is often divided into dry loading and wet loading. For dry loading, dissolve the substance to be separated and mix it in a small amount of silica gel G. After evaporating to dryness, fill it into the installed silica gel column, and then add chromatography solution for elution. For wet sample loading, silica gel G is first swollen with eluent and then loaded into the silica gel column, and then the dissolved sample is poured into the silica gel column for separation. There is little difference in the substance separation effect between these two methods. The wet sample loading operation is simple and convenient, but less polar solvents such as methylene chloride and ethyl acetate are used to dissolve the sample. For some substances with low solubility, it is advisable to use the dry method for sample loading.