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Thin layer chromatography

Thin layer chromatography

Chromatography is an important method for separation, purification and identification of organic compounds. It is applicable to the separation and purification of solid or liquid compounds. Its separation effect is far better than the general methods such as fractionation and recrystallization.


Separation principle of chromatography: based on the difference of physical and chemical properties of each component in the mixture, when a certain condition is selected to make each component flow through the supporting agent or adsorbent, each component is separated due to its different physical properties. Whether the chromatographic method can obtain satisfactory separation effect depends on the selection of conditions.

The following is the thin layer chromatography commonly used in organic laboratories.

Functions of thin layer chromatography:

Rapid separation of a small amount of substances can also be used as a test basis for selecting eluent for column chromatography

Qualitative analysis of a small amount of substances, such as "sample run" on the same thin layer plate, the reference standard can preliminarily identify whether the unknown compound is the same substance, which is often used in repeated experiments

Follow the reaction process, "run the sample" on the same thin-layer plate, and compare the reactants to track the reaction process and preliminarily judge whether there are new products

Use of thin layer chromatography:

The most typical method is to evenly spread a thin layer of adsorbent on the glass slide to make a thin layer plate, point the sample at the starting point with a capillary tube, place the thin layer plate in a chromatography tank containing solvent, take it out after the solution reaches the front, dry it, develop color, and determine the position of the color stain.