Micron Spherical Silica Gel Powder for Cosmetics Additive and Column Chromatography
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Column Chromatography – Principle, procedure, Applications

What is column chromatography?

Column chromatography is described as the useful technique in which the substances to be isolated are presented onto the highest point of a column loaded with an adsorbent (stationary phase), go through the column at various rates that rely upon the affinity of every substance for the adsorbent and the solvent or solvent mixture, and are typically gathered in solution as they pass from the column at various time.

The two most common examples of stationary phases for column chromatography are silica gel and alumina while organic solvents are regarded as the most common mobile phases.

Column Chromatography principle

The main principle involved in column chromatography is the adsorption of the solutes of the solution with the help of a stationary phase and afterward separates the mixture into independent components.

At the point when the mobile phase together with the mixture that requires to be isolated is brought in from the top of the column, the movement of the individual components of the mixture is at various rates.

The components with lower adsorption and affinity to the stationary phase head out quicker when contrasted with the greater adsorption and affinity with the stationary phase. The components that move rapidly are taken out first through the components that move slowly are eluted out last.

Column chromatography uses

Column chromatography is one of the versatile methods for purifying and separating both solids and liquids.

Major applications:

To isolate active constituents

To separate compound mixtures

To remove impurities or carry purification process

To isolate metabolites from biological fluids

To estimate drugs in drug formulations or crude extracts